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Corticotropin releasing hormone and related peptides can act as bioregulatory factors in human keratinocytes

  • Growth, Differentiation, and Senescence
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Summary

Following previous findings in human skin of the functional expression of genes for the corticotropin releasing hormone (CHR) receptor type 1 (CRH-R1) and CRH itself, we searched for local phenotypic effects for peptides related to CRH. We now report that CRH, sauvagine, and urocortin inhibit proliferation of human HaCaT keratinocytes in a dose-dependent manner. The peptides produced variable cyclic adenosine 3′∶5′-monophosphate stimulation with CRH having the highest potency. Binding of iodine 125 CRH to intact keratinocytes was inhibited by increasing doses of CRH, sauvagine, or urocortin, all showing equal inhibitory potency. Immunocytochemistry identified CRH-R1 immunoreactivity in HaCaT keratinocytes. In conclusion, CRH (exogenous or produced locally) and the related urocortin and sauvagine peptides can modify human keratinocyte phenotype through a receptor-mediated pathway.

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Authors and Affiliations

  1. Department of Pathology, Medical Center, Loyola University, 2160 First South Avenue, 60153, Maywood, Illinois

    Andrzej T. Slominski, Birgit Roloff & Jonathan Curry

  2. Department of Histology and Immunology, Medical University of Gdansk, Gdansk, Poland

    Blazej Zbytek

  3. Department of Pharmacology, School of Public Health, University of California, Berkeley, California

    Edward T. Wei

  4. Institute of Molecular Pharmacology, Berlin, Germany

    Klaus Fechner

  5. Department of Medicine, Southern Illinois University, Springfield, Illinois

    Jacobo Wortsman

Authors
  1. Andrzej T. Slominski
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  2. Birgit Roloff
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  3. Blazej Zbytek
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  4. Edward T. Wei
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  5. Klaus Fechner
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  6. Jonathan Curry
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  7. Jacobo Wortsman
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Correspondence to Andrzej T. Slominski.

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Slominski, A.T., Roloff, B., Zbytek, B. et al. Corticotropin releasing hormone and related peptides can act as bioregulatory factors in human keratinocytes. In Vitro Cell.Dev.Biol.-Animal 36, 211–216 (2000). https://doi.org/10.1290/1071-2690(2000)036<0211:CRHARP>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1071-2690(2000)036<0211:CRHARP>2.0.CO;2

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